Fig. 2.

DNA methylation in MT2 is impacted by early life experience and associated with Oxtr expression. a Offspring that received less parental care (MAN0) have increased DNA methylation across MT2 in the nucleus accumbens (13 male–female sibling pairs; MAN0: n = 7 males, 7 females; MAN1: n = 6 males, 6 females; mixed effect model with fixed effect of handling condition and random effects of individual and CpG site; Type II Wald F test F_(1,24) = 8.386, p = 0.008). Follow-up t tests between handling conditions at each site were conducted with Bonferroni correction; *p < 0.05, **p < 0.01. b DNA methylation in MT2 is associated with Oxtr expression in prairie vole nucleus accumbens. Spearman’s rank correlation was used to determine correlation. Sites with significant correlations (not corrected for multiple comparisons) are shown in color while nonsignificant sites are in gray. Spearman’s rho and p values for each site are in Additional file 1: Table S1. c DNA methylation in MT2 is associated with OXTR expression in human temporal cortex. Spearman’s rank correlation was used to determine correlation. Sites with significant correlations (not corrected for multiple comparisons) are shown in color while nonsignificant sites are in gray. Spearman’s rho and p values for each site are in Additional file 1: Table S2