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. 2021 Jan 22;14(1):dmm046417. doi: 10.1242/dmm.046417

Fig. 4.

Fig. 4.

Enlarged Rab7-positive endosomes contain secretory cargo proteins in retromer mutant cells. (A) Live-cell imaging of Rab7-EYFP (green) and mucins (Sgs3-DsRED; red) in control salivary glands pre-secretion. Rab7 localizes in small puncta that are completely distinct from mucins. (B) Live-cell imaging of Rab7 and mucins in Vps26 mutant salivary glands pre-secretion. Note that Rab7-positive endosomes are dramatically enlarged and contain mucin cargo proteins. (C) Live-cell imaging of Rab7 and mucins in control salivary glands post-secretion. Most mucins have been secreted and Rab7 localizes in small vesicles. (D) Live-cell imaging of Rab7 and mucins in Vps26 mutant salivary glands post-secretion. Unsecreted mucin-containing vesicles are dramatically enlarged and surrounded by Rab7. Scale bars: 5 µm.