Fig. 4.

AKT activity is required for Wnt signaling and NC induction. (A,B) Wild-type (A) or Wnt reporter (B) embryos were treated with 20 µM AKTi or DMSO from stage ∼10 to ∼12.5, and processed for in situ hybridization for the indicated markers (A) or imaged for eGFP expression (B). (C) Wnt reporter embryos were injected in one blastomere at the two-cell stage with dnAKT mRNA (50 pg), cultured to stage ∼12.5 and imaged for eGFP expression. Representative embryos are shown in dorsal view with anterior at the top. Injected side is indicated with a red asterisk. n, number of embryos scored. ***P<0.001 (χ² tests). Scale bars: 250 μm.