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. 2021 Jan 20;148(2):dev192658. doi: 10.1242/dev.192658

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Inhibition of BMP signaling after PAA progenitor specification induces no detectable defects of PAA morphogenesis. (A) Live confocal images of Tg(nkx2.5:ZsYellow;gata1:DsRed) embryos with DMSO or 10 μM DMH1 treatment for different durations. White arrowheads indicate the new sprouting clusters after removal of DMH1 at 38 hpf. Scale bar: 50 μm. (B) Expression analysis of nkx2.5 by in situ hybridization in embryos subjected to different treatments. The dotted lines represent the area of expression of nkx2.5. Black arrowhead indicates the newly emerged expression of nkx2.5 after removal of DMH1.