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. Author manuscript; available in PMC: 2021 Jul 15.

Published in final edited form as: Virology. 2020 Nov 25;553:135–153. doi: 10.1016/j.virol.2020.11.004

Fig. 6. — (A) The primary human fetal astrocytes (PHFA) were transduced with either Ad-Null or Ad-Agno WT and oxygen consumption rates (OCR) and OCR-related parameters were determined in the presence of a sequentially injected various mitochondrial inbitors (oligomycin, FCCP, antimycin A/rotenone) using a “Seahorse XF26 Extracellular Flux Analyzer” as described in materials and methods. (B) Ad-Agno-transduced and Ad-Null-transduced PHFA cells were analyzed by immunocytochemistry by probing the cells with a primary polyclonal α-Agno and a secondary fluorescein isothiocyanate (FITC)-conjugated goat α-rabbit antibodies using a fluorescence microscope as described under materials and methods. Scale: 22 μm. Non-Mito. Resp.: Non-Mitochondrial Respiration.

Fig. 6. — (A) The primary human fetal astrocytes (PHFA) were transduced with either Ad-Null or Ad-Agno WT and oxygen consumption rates (OCR) and OCR-related parameters were determined in the presence of a sequentially injected various mitochondrial inbitors (oligomycin, FCCP, antimycin A/rotenone) using a “Seahorse XF26 Extracellular Flux Analyzer” as described in materials and methods. (B) Ad-Agno-transduced and Ad-Null-transduced PHFA cells were analyzed by immunocytochemistry by probing the cells with a primary polyclonal α-Agno and a secondary fluorescein isothiocyanate (FITC)-conjugated goat α-rabbit antibodies using a fluorescence microscope as described under materials and methods. Scale: 22 μm. Non-Mito. Resp.: Non-Mitochondrial Respiration.