Figure 4. Relationships between functional connectivity 24 hr after the onset of cohabitation and social interaction during partner preference test in male and female prairie voles.

(A) Representation of a prairie vole brain with regions (nodes) that constitute the network with a significant negative association with the amount of social interaction during the PPT. Scatter-plot graphs (B–G) of the connections in a with best line fit between baseline functional connectivity (Fisher z-transformed partial-correlation values) and time on social incentive areas during cohabitation (percentage). The lower the connectivity between these regions at 24 hr of cohabitation, the longer the time spent on social incentive areas during the PPT. DG: dentate gyrus. LS: lateral septum. MeA: medial amygdala. MOB: main olfactory bulb. mPFC: medial prefrontal cortex. NAcc: nucleus accumbens. VP: ventral pallidum.

Figure 4—figure supplement 1. Relationships between social behaviors in the prairie vole.

Huddling latency was measured during the first hours of cohabitation, while percentage of time on social incentive areas and partner preference index were calculated from the partner preference test (PPT) evaluated between 48 hr or 72 hr after the onset of cohabitation. (A) No significant relationship was found between huddling latency and percentage of time on social incentive areas (rs(30) = −0.107; p=0.556), (B) between partner preference index and percentage of time on social incentive areas (rs(30)=0.89; p=0.627), (C) or between huddling latency and partner preference index (rs(30)=0.003; p=0.984) (N = 30). (D) Partner preference was neither reciprocated between male and female partners (rs(14) = −0.203; p=0.450) (N = 16). Sex = F: females; M: males.