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. 2021 Jan 14;10:e55081. doi: 10.7554/eLife.55081

Figure 5. Network-wide changes in time in brain functional connectivity of female and male prairie voles.

(A) NBR analysis via Linear mixed models (LMM) analysis results represented in a prairie vole brain with regions (nodes) comprising the brain network that undergoes significant changes in functional connectivity (Fisher z-transformed correlation values) after cohabitation with mating. Interregional connectivity (edges) is shown by color code. Red: increase of functional connectivity; blue: decrease of functional connectivity. ACC: anterior cingulate cortex. BLA: basolateral amygdala. dHIP: dorsal hippocampus. LS: lateral septum. mPFC: medial prefrontal cortex. NAcc: nucleus accumbens. RSC: retrosplenial cortex. vHIP: ventral hippocampus. VP: ventral pallidum. VTA: ventral tegmental area. (B–K) Functional connectivity values in violin plots showing full distribution of data and median. Connecting lines track longitudinal data of each subject between regions through specific MR acquisition time points (Session): Baseline, 24 hr, and 2 weeks of cohabitation. Color codes for data points and connecting lines distinguish male (cyan) from female subjects (pink). False discovery rate (FDR) post hoc significant differences are shown: *<0.05, **<0.01, ***<0.001.

Figure 5—source data 1. Functional connectivity values that changed significantly across sessions (Ses; 1, 2, and 3) in male and female prairie voles (females: F; males: M).
NA: data not available.
Figure 5—source data 2. Estimated marginal means (EMM) post hoc comparisons values obtained from the longitudinal analysis of functional connectivity data (edge), via linear mixed models with NBR.
Values are corrected for a false discovery rate (FDR) of q < 0.05 (PH_pFDR).

Figure 5.

Figure 5—figure supplement 1. Control regions for the longitudinal analysis of rsfMRI data.

Figure 5—figure supplement 1.

3D views of the nine control region masks embedded within the prairie vole template: primary auditory area (AUDp), the cerebellar cortex (CBX), forceps minor of the corpus callosum (fmi), laterodorsal thalamic nucleus (LD), primary motor area (MOp), motor-related medulla (MY), supplemental somatosensory a rea (SSs), primary visual area (VISp), and ventricle areas (Vent).
Figure 5—figure supplement 2. Ten components gICA.

Figure 5—figure supplement 2.

(a) Sensory/motor components, including motor cortex (IC01, IC07), somatosensory cortex (IC06), parietal cortex (IC01), colliculi (IC08), and olfactory bulb (IC09). (b) Default-mode network (IC04). (c) Salience network (IC02). (d) Striatum (IC03), (e) Hippocampus and entorhinal cortex (IC05, IC10).