Skip to main content
. 2021 Jan 26;13:743–755. doi: 10.2147/CMAR.S272851

Figure 4.

Figure 4

Circ_0031242 directly interacted with miR-924 through pairing to miR-924. (A) Schematic of the putative miR-924-pairing sites within circ_0031242 identified by CircInteractome online tool and the mutation in the target region. (B and C) Relative luciferase activity in Huh7-R and SNU-387-R cells cotransfected with circ_0031242 wild-type (circ_0031242 WT) or mutant-type (circ_0031242 MUT) luciferase reporter constructs and miR-924 mimic or miRNA NC mimic. (D and E) The enrichment levels of circ_0031242 and miR-924 in cell lysates incubated with anti-Ago2 or anti-IgG antibody. The expression of miR-924 by qRT-PCR in 30 healthy hepatic tissues, HCC tissues from 30 primary patients (DDP-sensitive) and 30 recurrent patients after treatment with DDP-based chemotherapy (DDP-resistant) (F), THLE-2, Huh7, SNU-387, Huh7-R and SNU-387-R cells (G), Huh7-R and SNU-387-R cells transfected with inhibitor NC or miR-924 inhibitor (H), Huh7-R and SNU-387-R cells transfected with si-NC, si-circ_0031242, si-circ_0031242+inhibitor NC or si-circ_0031242+miR-924 inhibitor (I). *P < 0.05 by a two-tailed Student’s t-test or ANOVA followed by Tukey-Kramer post hoc test.