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. 2021 Jan 26;13:743–755. doi: 10.2147/CMAR.S272851

Figure 6.

Figure 6

POU3F2 was directly targeted by miR-924. (A) Schematic of the miR-924-pairing sequence within the 3ʹUTR of POU3F2 mRNA and mutated the miR-924-pairing sites. (B and C) Relative luciferase activity in Huh7-R and SNU-387-R cells cotransfected with POU3F2 3ʹUTR wild-type (POU3F2-3ʹUTR WT) or mutant-type (POU3F2-3ʹUTR MUT) luciferase reporter construct and miR-924 mimic or miRNA NC mimic. POU3F2 mRNA and protein levels by qRT-PCR and Western blot in healthy hepatic tissues, HCC tissues from primary patients (DDP-sensitive) and recurrent patients after treatment with DDP-based chemotherapy (DDP-resistant) (D and E), THLE-2, Huh7, SNU-387, Huh7-R and SNU-387-R cells (F), Huh7-R and SNU-387-R cells transfected with pc-POU3F2 or pc-NC (G), Huh7-R and SNU-387-R cells transfected with miRNA NC mimic, miR-924 mimic, miR-924 mimic+pc-NC or miR-924 mimic+pc-POU3F2 (H). *P < 0.05 by a two-tailed Student’s t-test or ANOVA followed by Tukey-Kramer post hoc test.