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. 2021 Jan 26;13:743–755. doi: 10.2147/CMAR.S272851

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© 2021 Fan et al.

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The increased miR-924 level regulated DDP-resistant HCC cell behaviors by down-regulating POU3F2. Huh7-R and SNU-387-R cells were transfected with miRNA NC mimic, miR-924 mimic, miR-924 mimic+pc-NC or miR-924 mimic+pc-POU3F2 (POU3F2 overexpressing plasmid). (A) The IC₅₀ value of DDP in transfected cells by CCK-8 assay. (B) Cell viability by CCK-8 assay. (C and D) Cell migration and invasion by transwell assay. (E) Cell apoptosis by flow cytometry. (F) The levels of MRP1 and P-gp in transfected cells by Western blot. *P < 0.05 by ANOVA followed by Tukey-Kramer post hoc test.