Fig. 1.

Clinical, histological, and molecular parameters of H3F3A K27M mutated DMG with and without additional mutations in FGFR1. a Overview on all 83 analyzed cases with 12% of cases harboring BRAF or FGFR1 hotspot mutations. Percentages of characteristics refer to cases with known attribute only. Representative images of FGFR1 WT (b, c) and MU cases (d, e) demonstrate comparable histomorphology in both groups. T-SNE analysis of DMG reveals FGFR1 and BRAF MU cases to harbor similar DNA methylation profiles as FGFR1 and BRAF WT cases (f). FGFR1 MU cases showed a significantly better prognosis than FGFR1 WT cases (p = 0.023, g), and multivariate analyses confirmed significance of FGFR1 status independent of age and localization. WT* = wild type for respective hotspot, MU = mutant, n. a. = not available, *WHO grade of initial diagnosis