Skip to main content
. 2021 Jan 25;203(4):e00409-20. doi: 10.1128/JB.00409-20

FIG 6.

FIG 6

LafV negatively affects the expression of flagellar and nonflagellar genes in the surface-sensing regulon. Flagellar (F) and nonflagellar (NF) genes in the surface-sensing regulon are expressed in a hierarchy of three classes. (A) Strains bearing lux fusions in flagellar (fliH and flgM) and nonflagellar (VP1002) genes or a gene outside the surface-sensing regulon (VP0829) harboring either the vector (pLM1877) or a lafV+ expression plasmid (pLM3697). Reporter activity was measured at regular intervals during surface growth, and bars represent maximum reporter activities. Strains from left to right are LM12053, LM12054, LM12055, LM12056, LM12057, LM12058, LM12059, and LM12060. (B) The lafK::lacZ reporter strains LM10192 (WT) and LM7011 (lafK) harboring the vector (pLM1835) or an expression clone bearing lafV+ (pLM4710) or scrC+ (pLM3705) were grown on HI SW+ agar with 1 mM IPTG at 30°C and assayed for lacZ activity at 9 h. Strains from left to right are LM12044, LM12045, LM12046, LM12041, LM12042, and LM12043. For lux reporter fusions, values are the averages from 6 independent cultures, and lacZ reporters were measured in triplicate. In all panels, error bars represent standard deviations, and pairwise comparisons were performed between the indicated strains using Student’s t test (**, P < 0.01; ***, P < 0.001; NS, nonsignificant).