FIG 7.

LafV works positively and negatively to regulate lateral flagellar transcription. The laf::lux reporter strain LM1017 (flgE::lux) harboring the vector pLM1877 (LM12010) or the IPTG-inducible lafV⁺ expression clone pLM3697 (LM12011) was grown on a surface and monitored for light production using concentrations of IPTG of 0 μM (A), 6.25 μM (B), 25 μM (C), and 100 μM (D). Error bars represent standard deviations (n = 8). Pairwise comparisons of vector- and lafV⁺-expressing strains were performed at peak luminescence using Student’s t test (***, P < 0.001).