FIG 1.

Schematic representation of the L. monocytogenes eslABCR operon and interaction of the ABC transporter components EslABC. (A) Genomic arrangement of the eslABCR operon in L. monocytogenes. Arrows indicate the orientation of the genes. The small black arrow indicates the promoter identified in a previous study (20). (B) Model of the ABC transporter composed of the NBD protein EslA, which hydrolyses ATP, the TMD proteins EslB and EslC, and the cytoplasmic RpiR family transcription regulator EslR. The eslB gene (A) and EslB protein (B), which were investigated as part of the study, are in pink. (C) Interactions between the ABC transporter components. Plasmids encoding fusions of EslA, EslB, and EslC and the T18 and T25 fragments of the Bordetella pertussis adenylate cyclase were cotransformed into E. coli BTH101. Empty vectors pKT25 and pUT18C were used as negative controls, and pKT25-Zip and pUT18C-Zip were used as positive controls. Black lines indicate where lanes which were not required were removed. Self-interactions are marked with blue boxes and protein-protein interactions are marked with orange boxes. A representative image of three repeats is shown.