FIG 1.
Phosphosite T171 on PstP affects growth. (A) Schematic of the crystal structure of PstP from M. tuberculosis (PstPMtb) (55) (PDB code 1TXO). The threonine (T) sites on PstPMtb phosphorylated by the kinases PknA and PknB (56) are highlighted on the structure as follows: red, PstPMtb T137 (T134 in PstPMsmeg); blue, PstPMtb T141 (T138 in PstPMsmeg); and green, PstPMtb T174 (T171 in PstPMsmeg). (B) Doubling times of strains containing pstPMsmeg WT, phosphoablative mutant alleles pstPMsmeg T134A, T138A, and T171A, and phosphomimetic mutant alleles pstPMsmeg T134E, T138E, and T171E. Each dot is the mean of doubling times from two or three different experiments on different dates of a single isolated clone. The error bars represent the standard deviation. ***, P = 0.0003.