FIG 2.

PstP_Mtb dephosphorylates CwlM_Mtb. (A) Antiphosphothreonine (anti-P-Thr) and anti-His Western blots of in vitro phosphatase reactions with His-PstP_cWT_Mtb (top panel), His-PstP_cT174E_Mtb (middle panel), and no-phosphatase control (bottom panel) against phosphorylated His-SUMO-CwlM_Mtb. The assay was performed at least twice with two individually purified batches of each phosphatase; one set of images is shown here. (B) Quantification of relative intensities of anti-P-Thr over anti-His on Western blots. P values were calculated using two-tailed, unpaired t test. All the P values of WT versus T171E at any given time were nonsignificant. P values of WT versus T171E at 5 min = 0.683; 10 min = 0.809; 23 min = 0.934; 40 min = 0.831; 60 min = 0.876; and 90 min = 0.545. The error bars represent standard error of means.