Fig. 1.

Identification of novel 21-nt phasiRNAs biogenesis pathways in Oryza sativa. a OSsRNA-1 induced phasiRNAs generation from the transcript of LOC_Os01g57968.1 in seedling, b OSsRNA-2 induced phasiRNAs generation from the transcript of LOC_Os02g18750.1 in panicle, c osa-miR2118f induced phasiRNAs generation form the transcript of LOC_Os04g25740.1 in panicle, d OSsRNA-3 induced phasiRNAs generation from the transcript of LOC_Os05g43650.1 in seedling, e OSsRNA-4 induced phasiRNAs generation from the transcript of LOC_Os06g30680.1 in panicle, and f OSsRNA-4 induced phasiRNAs generation from the transcript of LOC_Os06g30680.1 in panicle (drought stress). For each graph, degradome supported cleavage signature on PHAS loci were profiled above, four high throughput degradome sequencing datasets (GSM434596, GSM455938, GSM455939 and GSM476257 which were represented by triangle, diamond, circle and square with different colors, respectively) were employed for scanning the sRNA triggers’ cleavage sites, which were marked by black arrows. The x axis represents the position on PHAS loci and the y axis represents the signature abundance. The abundance of 21-nt phasiRNAs which generated from the sense and antisense strand of PHAS loci in different samples were evaluated and profiled in middle images, the x axis represents the position on PHAS loci and the y axis represents the phasiRNA abundance. The phasing score of 21-nt PHAS windows were profiled at bottom, the x axis represents the position on PHAS loci and the y axis represents the phasing score