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. 2021 Jan 1;12(5):1483–1492. doi: 10.7150/jca.52395

Figure 1.

Figure 1

miR-361-3p was upregulated in liver T-ICs. A. The expression of miR-361-3p in CD24+ and CD24- HCC cells was assessed by real-time PCR assay. B. The expression of miR-361-3p in CD133+ and CD133- HCC cells was detected by real-time PCR assay. C. The expression of miR-361-3p in EpCAM+ and EpCAM- HCC cells was checked by real-time PCR assay. D. The expression of miR-361-3p in hepatoma spheroids was examined by real-time PCR assay. E. miR-361-3p expression in serial passages of hepatoma spheroids was analyzed by real-time PCR. F. The expression of miR-361-3p in CD24+ and CD24- primary HCC cells was assessed by real-time PCR assay. G. The expression of miR-361-3p in CD133+ and CD133- primary HCC cells was detected by real-time PCR assay. H. The expression of miR-361-3p in EpCAM+ and EpCAM- primary HCC cells was checked by real-time PCR assay. I. The expression of miR-361-3p in primary hepatoma spheroids was examined by real-time PCR assay. J&K. The correlation between the transcription level of miR-361-3p and CD24 or CD133 in fifty HCC tissues was determined by real-time PCR analysis. Data were normalized to U6 or β-actin as △Ct and analyzed by Spearman's correlation analysis.