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. 2021 Jan 1;12(5):1483–1492. doi: 10.7150/jca.52395

Figure 3.

Figure 3

miR-361-3p knockdown inhibited liver T-ICs self-renew and tumorigenesis. A. Huh7 and HepG2 cells were transfected with miR-361-3p sponge virus. The RNA was extracted and identified by real-time PCR. B. Representative images of hepatoma spheroids generated from Huh7/HepG2 miR-361-3p sponge and control cells. The number of spheroids was counted and compared. C. Flow cytometry analysis of CD24+ populations in spheroids derived from Huh7/HepG2 miR-361-3p sponge and control cells. Representative results from three independent experiments were shown. D. The expression of transcription factors (SOX2, OCT4 and c-Myc) in Huh7/HepG2 miR-361-3p sponge and control cells were detected by real-time PCR. E. The in vitro limiting dilution assay was used to check the population of liver T-ICs in Huh7/HepG2 miR-361-3p sponge and control cells. F. The in vivo limiting dilution assay was used to determine the tumorigenicity and liver T-ICs frequency. The frequency of tumor initiating cells was assessed using ELDA software.