Figure 2.

NL101 induces cell cycle arrest and apoptosis. A. Ramos and OCI-LY10 cells were treated with NL101 at indicated concentrations for 48h and then subjected to FACS analysis. B. Annexin-V labeling of Ramos and OCI-LY10 cells after treatment with indicated doses of NL101 for 48h and evaluated by flow cytometry. C. Dose- and time-responses of NL101 in Ramos and OCI-LY10 cells, cell lysates were used in western blot to detect proteins implicated in the DNA damage response, and GAPDH was determined as a loading control. D. Dose- and time-response changes of proteins involved in apoptosis after NL101 treatment of Ramos and OCI-LY10 cells. *, P < 0.05; **, P < 0.01.