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. 2021 Jan 15;11(7):3301–3316. doi: 10.7150/thno.51988

Figure 3.

Figure 3

RGD expressed on DMVs is required for their interactions with multiple cells in vitro. (A) Western blots of integrin αvβ3 and TLR4 on HUVECs, B16-F10 (melanoma cells), HL60 cells (neutrophils), and U937 cells (monocytes). (B) Conformation of the fusion protein ClyA-RGD-EGFP on the surface of bacteria simulated using Phyre2 software. (C) Flow cytometry (top) and its quantification (bottom) of cellular uptake of EGFP-DMVs and RGD-EGFP-DMVS by HUVECs, B16-F10, differentiated HL60 and differentiated U937 cells after the cells were incubated with DMVs at 12 µg/mL for 60 min. Data are represented as means ± SD, n = 3. *P < 0.05, * * P < 0.01, * * * P < 0.001.