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. 2021 Jan 15;11(7):3301–3316. doi: 10.7150/thno.51988

Figure 4.

Figure 4

RGD expression on DMVs is required for their cellular uptake by neutrophils and monocytes to mediate the transport of DMVs into tumor tissues. (A) Colocalization of RGD-EGFP-DMVs with tumor vasculature stained by anti-CD31, neutrophils stained by anti-LY-6G and monocyte/macrophages stained by F4/80 antibody. Alexa 488-anti-EGFP antibody was used to identify RGD-EGFP-DMVs. Arrows indicate the colocalizations of cells and DMVs. (B) Flow cytometric analysis of total cells of tumor tissues for DMVs positive (n = 5). (C and D) Flow cytometric analysis on the uptake of DMVs by neutrophils (anti-LY-6G) and monocytes/macrophages (anti-F4/80) (E and F) in tumor tissues (n = 5). 3 h after injection of DMVs, the tumor tissues were digested with collagenase type IV to obtain single cell suspensions. The flow cytometry data are in Figure S5. Data were expressed as means ± SD. *P < 0.05, * * P < 0.01, * * * P < 0.001 compared to PBS group unless specified otherwise.