Fig. 2.

Combined double ISH and immunostaining labelling for Pax3, Myf5 and HNK1. a Cross section of a stage HH20-21 labelled with Pax3 probe in blue (DIG) and Myf5 probe in red (FITC). The dermomyotome is labelled with Pax3 probe in blue (blue arrow in a). Myf5 transcripts are expressed in the myotome (red arrow in a). b Immunostaining is performed for HNK1 on the same cross section in a. HNK1 is detected in dorsal root ganglia, ventral roots and sympathetic ganglia (brown arrows in b). c Immunostaining is performed as in (b) but without primary antibody added. Cross section photos were taken at a magnification of 40×