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. 2020 Oct 10;155(1):145–155. doi: 10.1007/s00418-020-01920-7

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Fig. 4 — Combined double ISH and immunostaining labelling for Myf5, Sox10, HNK1 and Nkx2.2. a Cross section of a stage HH24-25 labelled with Myf5 probe in blue (DIG) and Sox10 probe in red (FITC). b Immunostaining is performed for HNK1 on the same cross section in (a). c Immunostaining was performed for Nkx2.2. d Cross section of the same stage labelled with Sox10 probe and stained for HNK1. HNK1 and Sox10 expression domains overlapped in the dorsal root ganglia and ventral roots. Note that Sox10 signals (red colour) are obscured by HNK1 (brown colour) signals. Ventromedial neural tube cells are labelled with Nkx2.2 (brown arrowhead in c)