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. 2021 Jan 18;7:610319. doi: 10.3389/fvets.2020.610319

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Copyright © 2021 Ouchi, Chowdhury, Cockrem and Bungo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental designs of this study. (A) Design of experiment 1. A closed arrow indicates the thermal conditioning treatment. Closed arrows indicate time points of sampling for collecting pectoral muscle. The total number of chicks was 32, with 16 chicks per group. The Pectoral muscle tissue was collected from half of the chicks in each group at 7 days old. Other Body weight was measured daily from 2 to 14 days of age in the other half of the chicks in each group. (B) Design of experiment 2. A closed arrow shows thermal conditioning and open arrows show heat challenge. The number of chicks was eight per group. (C) Design of experiment 3. + indicates treated and – indicates non-treated. Closed arrows show thermal conditioning treatment and open arrows indicate heat challenge. The number of chicks was eight per group. Four chicks were used for metabolome analysis in each group.