Figure 1.

High performance liquid chromatography (HPLC) analyses of α-glucosidase I (I) and II (II) and endo-β-N-acetylglucosaminidase (ENGase; III) activities of the microsomes obtained from pumpkin hypocotyls α-Glc’ase I and II activities were assayed using Glc₃Man₉GlcNAc₁-PA (G3M9’) and Glc2Man9GlcNAc1-PA (G2M9’), respectively, as substrates. The reaction mixtures were analyzed by size fractionation (SF)-HPLC, using a Shodex Asahipak NH2P-50 4E column. The ENGase activity was assayed using Man₆GlcNAc₂-PA (M6B) as a substrate and Man₃Xyl₁Fuc₁GlcNAc₂-PA (M3FX) as an internal standard. The reaction mixture was analyzed by reversed-phase (RP)-HPLC, using a Cosmosil 5C18-AR-II column.