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. 2021 Jan 18;11:610124. doi: 10.3389/fpls.2020.610124

Figure 2.

Figure 2

HPLC profiles of PA-oligosaccharides obtained from pumpkin microsomes. (I) The RP-HPLC profile of crude PA-oligosaccharides obtained from pumpkin microsomes is shown. The PA-oligosaccharides were separated by RP-HPLC using a Cosmosil 5C18-AR-II column. F1 contained GN1-FNGs, whereas F2 contained GN2-FNGs. (II) The SF-HPLC profiles of F1 and F2 obtained in (I). M9’, M8’, and M7’ indicate the elution positions of authentic PA-oligosaccharides (Man9GlcNAc1-PA, Man8GlcNAc1-PA, and Man7GlcNAc1-PA, respectively). M9 and M8 indicate the elution positions of authentic PA-oligosaccharides (Man9GlcNAc2-PA and Man8GlcNAc2-PA, respectively).