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. 2021 Jan 18;11:610124. doi: 10.3389/fpls.2020.610124

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Copyright © 2021 Katsube, Ebara, Maeda and Kimura.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Structural analysis of free N-glycans (FNGs) in pumpkin microsomes. (I) SF-HPLC of glycosidase-digested F1. 1, F1 obtained in Figure 1. 2, End-H-digested F1. 3, α-1,2-Man’ase-digested F1. M5’ indicates the elution position of authentic Man₅GlcNAc₁-PA. (II) SF-HPLC of glycosidase-digested F2. 1, F2 obtained in Figure 1. 2, End-H-digested F1. 3, α-1,2-Man’ase-digested F1. M5 indicates the elution position of authentic Man₅GlcNAc₂-PA.