Table 2.
The free radical scavenging capabilities in terms of IC50 values for ABTS and DPPH for the bee pollen hydrolysates produced using various enzyme types
| Enzyme | Free radical scavenging activity (IC50) (µg/mL)* | |||
|---|---|---|---|---|
| Control | 10 mg/mL | 25 mg/mL | 50 mg/mL | |
| ABTS assay | ||||
| Alcalase® | 27.99 ± 0.79f | 2.41 ± 0.06a | 3.64 ± 0.03ab | 2.81 ± 0.18a |
| Flavourzyme® | 35.46 ± 2.04g | 9.28 ± 0.25e | 5.67 ± 0.06bc | 6.56 ± 0.03cd |
| Neutrase® | 35.46 ± 2.04g | 8.51 ± 0.20de | 4.53 ± 0.07abc | 4.17 ± 0.16ab |
| DPPH assay | ||||
| Alcalase® | 50.55 ± 1.38f | 11.06 ± 0.30bc | 9.75 ± 0.03ab | 10.25 ± 0.13b |
| Flavourzyme® | 85.84 ± 0.67g | 14.50 ± 0.46d | 10.05 ± 0.13ab | 8.49 ± 0.25a |
| Neutrase® | 85.84 ± 0.67g | 16.60 ± 0.49e | 12.12 ± 0.29c | 9.77 ± 0.30ab |
*Results take the form of mean ± sem (n = 3). The different letters indicated as superscript show those items which have significant differences from others (p > 0.05)