Figure 4.

TAP-independent peptides are presented under inflammatory conditions

(A) TAP1, TAP2, SSR1, USP11, and VPS13B mRNA expression after 24 hr of IFN-γ stimulation was analyzed using three publicly available datasets and compared to the reference gene PPIA.

(B) TAP1, TAP2, SSR1, USP11, and VPS13B protein expression after 48 hr of IFN-γ stimulation was analyzed using publicly available MS data and compared to the reference protein PPIA (Megger et al., 2017). ND is not detected.

(C) HAP1 wild-type and TAP1 KO cells were treated with IFN-γ for 48 hr and surface HLA-A2 upregulation was measured by flow cytometry. Unstimulated (solid) or stimulated (dashed) wild type (blue) and TAP1 KO #1 (red) cells are shown, including unstimulated unstained wild type cells (gray).

(D) T cell coculture with unstimulated or IFN-γ stimulated wild-type or TAP1 KO clones (same as in Figure 1B). Culture supernatants were analyzed for the presence of cytokines using cytokine ELISA. Dotted lines represent the lower and upper detection limits of the ELISA. Repeated measures one-way ANOVA followed by Dunnett's (A), ordinary one-way ANOVA followed by Dunnett's (B) or by Sidak's (D) multiple comparisons test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, ns is not significant.

See also Figure S1.