Fig. 2. Gene expression analysis of mict mutants and WT cucumber plants.

a Trichome morphological observations in the 5 stages on the first leaf at 24 h, 32 h, 40 h, 48 h, and 56 h after germination. b qRT-PCR assay of Mict expression in different organs. Panels (a–f) indicate significant differences by Tukey’s multiple comparisons test with P < 0.05 between different samples. (c–f) RNA in situ hybridization of Mict in WT, with (f) showing the sense-probe and (c–e) as the antisense-probe and fruits at 1 cm, 1.5 cm, and 2 cm. qRT-PCR results are shown in (b). Scale bars indicate 1 mm in (a) and 200 μm in (c–f)