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. 2021 Jan 18;7:624776. doi: 10.3389/fcvm.2020.624776

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Copyright © 2021 Fiegle, Schöber, Dittrich, Cesnjevar, Klingel, Volk, Alkassar and Seidel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal microscopic images and 3D t-system reconstruction of LV tissue samples from pediatric patients with AVSD (left) or fulminant myocarditis (right). (A,B) Raw confocal images of myocardial tissue sections stained with WGA. (C,D) The surface membrane (white) and t-system (red, green) were discerned by image segmentation. Reconstructions represent three-dimensional views of the highlighted areas, with surface membranes (gray) and the t-system. Scale bars in (A,C) also apply to (B,D), respectively.