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. 2021 Jan 18;8:617758. doi: 10.3389/fcell.2020.617758

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Copyright © 2021 Liao, Wu, Wang, Wang, Wang, Gao, Zhong, Ma, Wu and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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OTUB1 promotes and regulates the expression of Cyclin E1. (A) The relationship between OTUB1 and Cyclin E1 was analyzed via gene MANIA database. (B) The protein expression of Cyclin E1 in BPH, ADPC, and CRPC was detected by IHC staining. (C) Western blotting detecting the expression of Cyclin E1 in PC3 and C4-2 cells transfected with otub1 overexpression and otub1 c91s. (D) Western blotting detecting the expression of Cyclin E1 in PC3 and C4-2 cells transfected with OTUB1 siRNA. (E) Western blotting detecting the expression of Cyclin E1 in PC3 and C4-2 cells transfected with an increasing gradient OTUB1.