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. 2021 Jan 18;8:617758. doi: 10.3389/fcell.2020.617758

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Copyright © 2021 Liao, Wu, Wang, Wang, Wang, Gao, Zhong, Ma, Wu and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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OTUB1 rescues Cyclin E1 from proteasomal degradation. (A) Western blotting detecting the expression of Cyclin E1 in PC3 cells transfected with OTUB1 siRNA and treated with 10 uM MG132 for 8 h. (B) Western blotting detecting the expression of Cyclin E1 in PC3 cells transfected with OTUB1 siRNA and treated with CHX and chloroquine for 8 h. (C,D) Western blotting detecting the expression of Cyclin E1 in PC3 cell transfected OTUB1 siRNA and treated with CHX for 0, 4, 8, and 24 h. (E) OTUB1 interacted with Cyclin E1 (upper panel) and Cyclin E1 interacted with OTUB1 (lower panel) were found via immunoprecipitation assay. (F) The effect of OTUB1 knockdown on ubiquitination of Cyclin E1 in PC3 cells. (G) The effect of increased OTUB1 on ubiquitination of Cyclin E1 in PC3 cells. (H) The correlation between OTUB1 and Cyclin E1 was analyzed online (R = 0.19, P < 0.01).