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. 2021 Jan 18;8:617758. doi: 10.3389/fcell.2020.617758

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Copyright © 2021 Liao, Wu, Wang, Wang, Wang, Gao, Zhong, Ma, Wu and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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OTUB1/Cyclin E1 axis promotes the proliferation of PCa cells. (A) Western blotting detecting the expression of Cyclin E1 in PC3 cell transfected with OTUB1 overexpression and Cyclin E1 siRNA. (B,D) MTT assays and colony formation assays in PC3 cell transfected with OTUB1 overexpression and Cyclin E1 siRNA. (C) Transwell assay in PC3 cell transfected with OTUB1 overexpression and Cyclin E1 siRNA. (E) Western blotting detecting the expression of Cyclin E1 in PC3 cell treated with a different dose of RO-3306. (F,H) MTT assays and colony formation assays in PC3 cell treated with a different dose of RO-3306. (G) Transwell assay in PC3 cell treated with a different dose of RO-3306. ***P < 0.001; ****P < 0.0001.