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. 2021 Jan 18;11:622513. doi: 10.3389/fpls.2020.622513

FIGURE 4.

FIGURE 4

Transient LsPDI1 expression induces Jasmonic acid (JA)-mediated plant defense responses. (A,B) Detection of callose deposition induced by transient LsPDI1-GFP fusion protein expression in Nicotiana benthamiana leaves at 48 h (n = 6). GFP was used as the control. Callose deposition was detected by aniline blue staining. Bar = 50 μm. The red arrow indicates the spots of callose deposition. (C–F) Relative mRNA expression of NbPR1 (salicylic acid [SA]) and NbPR2 (SA), NbPR3 (JA), and NbPR4 (JA) induced by transient LsPDI1-GFP fusion protein expression in Nicotiana benthamiana leaves at 24 and 48 h (n = 5). (G–J) Relative mRNA expression of OsPAL (SA), OsPR1a (SA), OsAOS2 (JA), and OsPR4 (JA) induced by transient LsPDI1-GFP fusion protein expression in rice protoplast at 12 h and 24 h (n = 5). “**” indicates significant differences between treatments (P ≤ 0.01; Student’s t-test). (K–M) The JA (K), JA-Ile (L), and SA (M) hormone levels in the Nicotiana benthamiana leaves of transiently expressing LsPDI1 or GFP at 24 and 48 h (n = 5). “**” indicates significant differences between treatments (P ≤ 0.01; Student’s t-test).