FIGURE 5.

Transient LsPDI1 overexpression in planta decreases insect performance on host plant. (A–C) Spodoptera frugiperda or Myzus persicae have a greater preference for feeding on Nicotiana benthamiana leaves transiently overexpressing GFP than for those overexpressing LsPDI1. After 24 h of transient foliar LsPDI1 or GFP expression, eight second-instar Spodoptera frugiperda larvae or 15 newly emerged Myzus persicae were set in the middle of harvested leaves and the numbers of them on leaves were recorded at 12 and 24 h after feeding, respectively (n = 18). (D) Transient LsPDI1 overexpression decreased Myzus persicae fecundity on host plants. Agroinfiltration and aphid assays were performed side-by-side using LsPDI1-GFP and GFP. Average number of nymphs produced per adult is based on three replicates. Each replicate consisted of six replicated leaf disks (n = 18). “**” indicates significant difference between treatments (P ≤ 0.01, Student’s t-test).