FIGURE 6.

Determination of functional LsPDI1 domains causing cell death. (A) Functional domains of LsPDI1 have a N-terminal signal peptide for secretion, four thioredoxin domains (a, b, b’, and a’) predicted by an NCBI conserved domain search, and two catalytic domains containing characteristic CGHC active sites in the a and a’ domains. (B) Left panel indicates the amino acid positions for initiation and termination and schematic view of LsPDI1 deletion mutants. Right panel indicates the cell-death lesions on Nicotiana benthamiana leaves expressing LsPDI1 deletion mutants. Symptoms were photographed at 72 h after infiltration (n = 3). SP indicates signal peptide. ER indicate retention signal of endoplasmic reticulum HEEL. “ + ” and “–” indicate presence and absence of cell death symptoms, respectively.