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. 2021 Jan 18;11:622513. doi: 10.3389/fpls.2020.622513

FIGURE 7.

FIGURE 7

Determination of PDI1 conservation among the three planthopper species. (A) Alignment of amino acid sequences of LsPDI1 and its homologs based on the brown planthopper (BPH) and white-backed planthopper (WBPH) without the signal peptide. Identical amino acid residues are highlighted by pink ground shading. Similar amino acid residues are shaded with a light blue background. Different amino acids residues are not shaded. The four thioredoxin domains (a,b,b’, and a’) were indicated using an NCBI conserved domain search software. Active CGHC motif sequences are boxed in the a and a’ domains. (B) Determination of reactive oxygen species (ROS) burst symptoms caused by transiently expressing LsPDI1 and its homologs from BPH and WBPH in Nicotiana benthamiana (n = 6). GFP was the negative control. Symptoms were photographed at 12 h after infiltration. (C) Determination of cell death symptoms caused by transiently expressing LsPDI1 and its homologs from BPH and WBPH in N. benthamiana (n = 6). GFP was the negative control. Symptoms were photographed at 60 h after infiltration.