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. 2021 Jan 18;8:619924. doi: 10.3389/fcell.2020.619924

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Copyright © 2021 Tang, Zhang, Lin, Xu, Chen, Wang, Zhang, Tang, Shi, Liu, Yang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemical staining of beclin1/LC-3I/II during the development of corpus luteum. Three time points (Day 1, 4, and 10 after pregnancy) were selected and represented as the developmental process of corpus luteum according to our previous reports. Ovarian sections of each point (A,D,G,J,M for Day 1; B,E,H,K for Day 4; C,F,I,L,O for Day 10) were immunostained for beclin1/LC-3I/II and counterstained with hematoxylin. The beclin1/LC-3I/II immunohistochemical signals appear brown, and the background counterstaining appears blue (A–F for LC-3I/II and G–L for Beclin1). Negative control remained unstained, lacking primary antibody instead of serum (M–O). CL, corpus luteum, bar = 100 μm.