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. 2021 Jan 18;8:608951. doi: 10.3389/fbioe.2020.608951

Figure 1.

Figure 1

Impact of substrate mechanics and cytoskeleton on TRPV4-mediated currents. (A) Schematic representation of pillar array experiment. Cells are cultured on deformable arrays and stimuli are applied at cell-substrate interface by deflecting a pilus subjacent to the cell using a glass probe (in blue). The resulting channel activity is concurrently monitored using whole-cell patch-clamp. Bottom panels show bright-field images of the substrates R3, R2 and R2B, scale bar 10μM. (B) Representative traces of TRPV4-WT mediated currents in response to increasing deflections from 28 to 212 nm on the R3 substrate. (C) Stimulus-response plot for TRPV4-WT cells cultured on R3 and R2 substrates (ordinary two-way ANOVA, **p = 0.009) (D) Stimulus-response plot for TRPV4-WT cells cultured on R2, R2 stiff and R2B (ordinary two-way ANOVA, *p = 0.019). Stimulus-response plots for TRPV4-WT cultured on R3 substrate after treatment with (E) 2.5 μM cytochalasin D and (F) 10 μM nocodazole compared to the control (ordinary two-way ANOVA, *p = 0.01). Data are presented in mean ± s.e.m, number in brackets indicates number of cells.