Figure 5.

Mechanically activated currents in TRPV4 mutants associated with arthropathy in response to pillar deflection. Stimulus-response plots for (A) TRPV4-G270V, (B) TRPV4-R271P and (C) TRPV4-F273L in comparison to TRPV4-WT expressing cells cultured on R3 substrate. Data were analyzed using ordinary two-way ANOVA with Sidak's multiple comparisons. The first three bins in TRPV4-G270V curve (1–10, 10–50, and 50–100 nm) were analyzed separately for Type I vs. Type II as indicated in the dashed box. *p = 0.045 and ****p < 0.0001 in (A) (TRPV4-G270V, Type I = 10, Type II = 12 stimulation points). Data are presented as mean ± s.e.m., number in brackets indicates number of stimulation points. Full cell numbers outlined in Supplementary Table 1.