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. 2021 Jan 18;11:577237. doi: 10.3389/fphys.2020.577237

FIGURE 3.

FIGURE 3

Acacetin inhibited the expression of inflammatory mediators in vitro. Effects of acacetin on cell viability (A) and the secretion of nitric oxide (NO) (B) in lipopolysaccharide (LPS)-induced RAW264.7 cells. (C) Protein expression levels of LPS-induced RAW264.7 cells, including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), were determined by western blot analysis. (D) Quantification of protein expression was determined by densitometric analysis of the blots. Expression was normalized to β-actin. (E) Effects of acacetin on the expression of proinflammatory mediator mRNA in LPS-induced RAW264.7 cells. The mRNA expression of COX-2 and iNOS were determined by qPCR. All expressions were normalized to β-actin. Data are presented as the mean ± SEM (n = 3). *p < 0.05, ***p < 0.001 vs. the DSS group; ###p < 0.001 vs. the vehicle group.