Skip to main content
. 2021 Jan 18;11:540591. doi: 10.3389/fphys.2020.540591

FIGURE 5.

FIGURE 5

ACE2 is a direct transcriptional target of ATF3. (A) The sequence logo of a potential ATF3 binding site in JASPAR. (B) The relative luciferase activities of the full ACE2 promoter and the truncated constructs in HEK-293T cells. *P < 0.05 vs. pGL3.10-Basic vector. (C) The relative luciferase activities of the full ACE2 promoter P in vascular smooth muscle cells under mechanical stretch for 6 h or not. *P < 0.05 vs. static control. (D,E) The relative luciferase activities of the full ACE2 promoter P co-transfected with pcDNA-ATF3 (or ATF3 siRNA). *P < 0.05 vs. control. (F) ChIP assay showing ATF3 directly bound to the ACE2 promoter (P6, at the -1,296 to -1,073 bp region). IgG, negative control; H3, positive control; ATF3, ATF3 antibody. (G,H) Luciferase activity analysis of P-P7 promoter with or without co-transfection of ATF3. #P < 0.05 vs. the corresponding promoter without co-transfection of ATF3 (n = 4).