Figure 2.

Knockdown of GACAT3 inhibited cell proliferation. Cell proliferation was measured by CCK-8 assay. After transfection of CACAT3 CRISPR-Cas13 or negative control CRISPR-Cas13, OD values were measured and converted to cell numbers. ANOVA was used for the comparison of curves of cell proliferation. (A) Inhibition of cell proliferation was observed in bladder cancer 5637 cells (**p < 0.01). (B) Inhibition of cell proliferation was observed in bladder cancer T24 cells (**p < 0.01). Data are shown as mean ± SD. Each experiment in both cell lines was performed in double for three independent times.