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Knockdown of GACAT3 induced apoptosis. Forty-eight hours after transfection of GACAT3 CRISPR-Cas13 or negative control CRISPR-Cas13, the cell apoptosis changes were determined by ELISA. Cell apoptosis induction was observed in GACAT3 CRISPR-Cas13-transfected bladder cancer 5637 (***p < 0.01) and T24 (***p < 0.01) cells using ELISA. Data are shown as mean ± SD. Each experiment in both cell lines was performed in triplicate for three independent times.
