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Knockdown of GACAT3 increased p21, Bax, and E-cadherin protein expression. After transfection of GACAT3 CRISPR-Cas13 or negative control CRISPR-Cas13, western blot assay was used to detect expression changes of p21, Bax, and E-cadherin in bladder cancer cells. (A) Representative images of western blot assay in 5637 cells. (B) Representative images of western blot assay in T24 cells.
