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. 2019 Jul 12;27(7):779–788. doi: 10.3727/096504018X15442985680348

Figure 3.

Figure 3

PAR2 coordinates with TGF-β to mediate 5-FU-triggered apoptosis and cell migration. (A) HCT116 cells were treated with 50 μg/ml of 5-FU after pretreatment for 1 day with 100 nmol/L LY2157299 and 100 U/ml trypsin, and then the expression of target proteins was analyzed through immunoblotting. (B) HCT116 cells were processed as in (A), and then cell survival was analyzed using the MTS assay. (C) HCT116 cells were processed as in (A), after which caspase 3/7 activity was analyzed. (D) HCT116 cells were processed as in (A), and the migration of the surviving cells after treatment was analyzed. (E) HCT116 cells transfected with pcDNA3.1 (control+) or pcDNA3.1-PAR2 (PAR2+) were treated with 50 μg/ml of 5-FU after pretreatment for 1 day with 100 nmol/L LY2157299, and then the expression of target proteins was analyzed through immunoblotting. (F) HCT116 cells were processed as in (E), and then cell survival was analyzed using the MTS assay. (G) HCT116 cells were processed as in (E), after which caspase 3/7 activity was analyzed. (H) HCT116 cells were processed as in (E), and the migration of the surviving cells after treatment was analyzed. *p < 0.05, **p < 0.01.