Figure 6.
PAR2 mediates the 5-FU tumor-killing effect and inhibition of cell migration in vivo. (A) Nude mice were subcutaneously injected with 1 × 106 wild-type HCT116 cells and, after 7 days, were intraperitoneally injected, once every 2 days for 10 days, with 50 mg/kg of 5-FU, 1 mg/kg of ENMD-547, or their combination. Tumor volume at specific times after treatment was evaluated; n = 5 per group. (B–D) Mice with HCT116 xenograft tumors were treated as in (A) for 4 consecutive days, and then tissue sections were stained for active caspase 3 (B), vimentin (C), and E-cadherin (D). **p < 0.01.