Figure 5.
ELAVL1 was a direct target of miR-324-5p. (A) Predicted miR-324-5p target sequences in the 3′-untranslated region (3′-UTR) of ELAVL1. (B) The miR-324-5p target sequence from ELAVL1 was cloned into the 3′-UTR of a luciferase reporter gene. Seed site mut was used to control for binding specificity. Luciferase activity was determined by the Dual-Luciferase Reporter Assay System. *p < 0.05 versus NC group.